Given the placement of this ALP on the deck of the Biomek NX, the entire process of protein purification and quantitation was performed in a complete walk-away automated format. The AD 340 Automated Labware Positioner (ALP), an integrated absorbance reader, was used for absorbance measurements in the Bradford assay.
Following purification, a Bradford assay was used to determine the quantitative yields of the His-tag protein products in each of the aliquots from the purified samples. For proteomic applications, His-tag proteins were purified from bacterial cultures in a 96-well plate format. In this case, the system quickly and efficiently prepared clones for rapid identification of target sequences. Additionally, the plasmid preparation method was used to purify plasmid DNA from bacterial clones isolated in a bacterial two-hybrid screening procedure.
Homan La Roche, Ltd.), and capillary sequencing. DNA quantity and quality were assessed by spectrophotometric analysis, restriction digestion, PCR (The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. A single plate of bacterial pellets can be processed to purified plasmid DNA without user interaction after initial setup. For genomic applications, methodology for plasmid DNA purification using Promega Wizard® SV 96 reagents was developed for the Biomek NX. This technical paper describes the utilization of a new automated liquid handler from Beckman Coulter, Inc., the Biomek® NX Laboratory Automation Workstation, for genomic and proteomic applications.
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